Investigation of the Change of pH Affecting Enzyme Activity

Introduction:Research Question:The purpose of this experiment is to check a factor simulateing enzyme activity. Hypothesis:A change in the pH of hydrogen peroxide will have an impact on enzyme activity. Explanation of Hypothesis:Enzymes are catalytic world-wide proteins made by living things that regulate the movement of molecules with metabolic pathways, as well as speed up chemical responses without being changed. They speed up metabolic reactions by heavy(a) energy barriers. Catalyst is a chemical factor that changes the straddle of a reaction without being consumed by the reaction (IB biological science notes). Enzymes generally function best at a certain(p) pH. The optimum pH changes depending on the enzyme in question. The enzyme experimented on in this experiment was the enzyme catalase, which works best at a pH of 7.0 (http://www.worthington-biochem.com/IntroBiochem/effectspH.html). If the pH of the substrate is changed even slightly the enzyme may arrest den aturation (IB biology notes). Denatuation is the changing of the structure of an enzyme so that it stack no longer carry out its function, this is usually unchanging (IB biology notes). Variables:Dependent variable:Rate of catalase reaction when dropped into a nominate vial containing 20ml of hydrogen peroxide at common chord different pH levels. Independent variable:The pH of a 20ml clear vial of hydrogen peroxide. Controlled variable:Temperature exclusively tests were taken in the same room, at room temperature.

Time on the whole tests were taken on the same day, in the same 90 minute lab session. Light intensit y All tests were taken under the same light ! intensity. Concentration of solutions All 3rd 20ml vials of hydrogen peroxide came from the same batch. Source of biological material used All materials were taken from the same batch. Total raft of reaction mixtureAll liquids were poured from the same 10ml graduated cylinder. Materials:Notebook or sea gull paperPen or pencil3 clear vials2 20ml hospital plastic cup100% Catalase solution, If you want to admit a full essay, order it on our website: OrderCustomPaper.com

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